Glutathione-S-transferase-pi (GST-pi) expression in renal cell carcinoma
Main Article Content
Keywords
Glutathione-s-transferase, GST-pi, immunohistochemistry, multidrug resistance, renal cell carcinoma
Abstract
Multidrug resistance correlates with unfavourable treatment outcomes in numerous cancers including renal cell carcinoma. The expression and clinical relevance of Glutathione-S-transferase-pi (GST-pi), a multidrug resistance factor, in kidney tumors remain controversial. We analyzed the expression of GST-pi in 60 formalin-fixed, paraffin-embedded renal cell carcinoma samples by immunohistochemistry and compared them with matched normal regions of the kidney. A significantly higher expression of GST-pi was observed in 87% of clear cell carcinoma and 50% of papillary subtypes. GST-pi expression did not correlate with tumor grade or patient survival. GST-pi is unlikely to be a prognostic factor for renal cell carcinoma. However, further studies with large number of samples are warranted to establish the role of GST-pi, if any, in intrinsic or acquired resistance of renal cell carcinoma to conventional treatments.
References
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18. Yang X, Long S, Deng J, Deng T, Gong Z, Hao P. Glutathione S-transferase polymorphisms (GSTM1, GSTT1 and GSTP1) and their susceptibility to renal cell carcinoma: an evidence-based meta-analysis. PLoS One. 2013;8(5):e63827. Doi: http://dx.doi.org/10.1371/journal.pone.0063827
19. Harrison DJ, Kharbanda R, Cunningham DS, McLellan LI, Hayes JD. Distribution of glutathione S-transferase isoenzymes in human kidney: basis for possible markers of renal injury. J. Clin. Pathol. 1989;42 (6): 624–8. Doi: http://dx.doi.org/10.1136/jcp.42.6.624
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21. Angus R, Lai T, Collins C, Stonebridge B, Symes M. Expression of glutathione-s-transferase isotypes in relation to Doxorubicin sensitivity and prognosis in patients with renal-carcinoma. Int J Oncol. 1994;4(6):1383-6. [PMID: 21567066]
2. Porta C, Sabbatini R, Procopio G, Paglino C, Galligioni E, Ortega C. Primary resistance to tyrosine kinase inhibitors in patients with advanced renal cell carcinoma: state-of-the-science. Expert Rev Anticancer Ther. 2012;12(12):1571-7. Doi: http://dx.doi.org/10.1586/era.12.81
3. Busch J, Seidel C, Weikert S, Wolff I, Kempkensteffen C, Weinkauf L, Hinz S, Magheli A, Miller K, Grunwald V. Intrinsic resistance to tyrosine kinase inhibitors is associated with poor clinical outcome in metastatic renal cell carcinoma. BMC Cancer. 2011;11:295. Doi: http://dx.doi.org/10.1186/1471-2407-11-295
4. Ravaud A, Gross-Goupil M. Overcoming resistance to tyrosine kinase inhibitors in renal cell carcinoma. Cancer Treat Rev. 2012;38(8):996-1003. Doi: http://dx.doi.org/10.1016/j.ctrv.2012.01.003
5. Townsend DM, Tew KD. The role of glutathione-S-transferase in anti-cancer drug resistance. Oncogene. 2003;22(47):7369-75. Doi: http://dx.doi.org/10.1038/sj.onc.1206940
6. Sun HD, Ru YW, Zhang DJ, Yin SY, Yin L, Xie YY, Guan YF, Liu SQ. Proteomic analysis of glutathione S-transferase isoforms in mouse liver mitochondria. World J Gastroenterol. 2012;18(26):3435-42.
Doi: http://dx.doi.org/10.3748/wjg.v18.i26.343
7. Yu DS, Ma CP, Chang SY. Establishment and characterization of renal cell carcinoma cell lines with multidrug resistance. Urol Res. 2000;28(2):86-92. Doi: http://dx.doi.org/10.1007/s002400050143
8. Mizutani Y, Bonavida B, Koishihara Y, Akamatsu K, Ohsugi Y, Yoshida O. Sensitization of human renal cell carcinoma cells to cis-diamminedichloroplatinum(II) by anti-interleukin 6 monoclonal antibody or anti-interleukin 6 receptor monoclonal antibody. Cancer Res. 1995;55(3):590-6. [PMID: 7834629]
9.Tanaka T, Nishiyama Y, Okada K, Satoh K, Fukuda A, Uchida K, Osawa T, Hiai H, Toyokuni S. Over-expression of glutathione S-transferase Yp isozyme and concomitant down-regulation of Ya isozyme in renal cell carcinoma of rats induced by ferric nitrilotriacetate. Carcinogenesis.1998;19(5):897-903. Doi: http://dx.doi.org/10.1093/carcin/19.5.897
10. Ahn H, Lee E, Kim K, Lee C. Effect of glutathione and its related enzymes on chemosensitivity of renal cell carcinoma and bladder carcinoma cell lines. J Urol. 1994;151(1):263-7. [PMID: 8254825]
11. Grignon DJ, Abdel-Malak M, Mertens WC, Sakr WA, Shepherd RR. Glutathione S-transferase expression in renal cell carcinoma: a new marker of differentiation. Mod Pathol. 1994;7(2):186-9. [PMID: 8008741]
12. Klone A, Weidner U, Hussnatter R, Harris J, Meyer D, Peter S, Ketterer B, Sies H. Decreased expression of the glutathione S-transferases alpha and pi genes in human renal cell carcinoma. Carcinogenesis. 1990;11(12):2179-83. Doi: http://dx.doi.org/10.1093/carcin/11.12.2179
13.Di Ilio C, Aceto A, Bucciarelli T, Angelucci S, Felaco M, Grilli A, Zezza A, Tenaglia R, Federici G. Glutathione transferase isoenzymes in normal and neoplastic human kidney tissue. Carcinogenesis. 1991;12(8):1471-5. Doi: http://dx.doi.org/10.1093/carcin/12.8.1471
14. Oudard S, Levalois C, Andrieu JM, Bougaran J, Validire P, Thiounn N, Poupon MF, Fourme E, Chevillard S. Expression of genes involved in chemoresistance, proliferation and apoptosis in clinical samples of renal cell carcinoma and correlation with clinical outcome. Anticancer Res. 2002;22(1A):121-8. [PMID: 12017273]
15. Volm M, Kastel M, Mattern J, Efferth T. Expression of resistance factors (P-glycoprotein, glutathione S-transferase-pi, and topoisomerase II) and their interrelationship to proto-oncogene products in renal cell carcinomas. Cancer. 1993;71(12):3981-7.[PMID:8099529]
16. Toffoli G, Viel A, Tumiotto L, Giannini F, Volpe R, Quaia M, Boiocchi M. Expression of glutathione-S-transferase-pi in human tumours. EurJCancer.1992;28A(8-9):14416. [PMID:1515267]
17. Harrison DJ, Kharbanda R, Bishop D, McLelland LI, Hayes JD. Glutathione S-transferase isoenzymes in human renal carcinoma demonstrated by immunohistochemistry. Carcinogenesis. 1989;10(7):1257-60.
Doi: http://dx.doi.org/10.1093/carcin/10.7.1257
18. Yang X, Long S, Deng J, Deng T, Gong Z, Hao P. Glutathione S-transferase polymorphisms (GSTM1, GSTT1 and GSTP1) and their susceptibility to renal cell carcinoma: an evidence-based meta-analysis. PLoS One. 2013;8(5):e63827. Doi: http://dx.doi.org/10.1371/journal.pone.0063827
19. Harrison DJ, Kharbanda R, Cunningham DS, McLellan LI, Hayes JD. Distribution of glutathione S-transferase isoenzymes in human kidney: basis for possible markers of renal injury. J. Clin. Pathol. 1989;42 (6): 624–8. Doi: http://dx.doi.org/10.1136/jcp.42.6.624
20. J. Charles Jennette. The kidney. Malignant tumors of the kidney. In: Raphael Rubin, David S.Strayer. Rubin's Pathology: Clinicopathologic Foundations of Medicine, 5th Edition. Philadelphia: Lippincott Williams &Wilkins, a Wolters Kluwer business; 2008. p.743.
21. Angus R, Lai T, Collins C, Stonebridge B, Symes M. Expression of glutathione-s-transferase isotypes in relation to Doxorubicin sensitivity and prognosis in patients with renal-carcinoma. Int J Oncol. 1994;4(6):1383-6. [PMID: 21567066]
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Feb 22, 2015
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Glutathione-S-transferase-pi (GST-pi) expression in renal cell carcinoma. (2015). Journal of Kidney Cancer, 2(1), 25-29. https://doi.org/10.15586/jkcvhl.2015.22
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Kidney Cancer: Original Articles
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How to Cite
Glutathione-S-transferase-pi (GST-pi) expression in renal cell carcinoma. (2015). Journal of Kidney Cancer, 2(1), 25-29. https://doi.org/10.15586/jkcvhl.2015.22